Determination of ascorbic acid in different citrus fruits under reversed phase conditions with UPLC

This study compared the concentration of ascorbic acid between citrus fruits collected from the farm of Kerman in Iran. Determination of ascorbic acid was carried out using a liquid chromatography coupled to a diode array detector, with reverse phase and isocratic elution. The validation parameters showed efficiency, adequate linearity, relative standard deviation values 0.02 % (n=10) for repeatability and 0.5 % (n=15) for reproducibility, limit of detection (LOD) was 0.2 mg Lrecovery was between 97.3 % and 103.6%. Ascorbic acid was determined for 10 species of citrus: sour and sweet orange, umbilical orange, novel orange, lime, lemon, pink and white grapefruit, aeglemarmelos, bergamot, sour and sweet tangerines and clementine. The average ascorbic acid was the highest in Shahdad’s citrus fruits.